Immune Regulation in Cancer: Capturing Signaling Patterns through Global Phosphorylation Profiling

Mass spectroscopy-based proteomics approaches can be used to understand changes in intracellular signaling pathways in disease states and immune activation. This presentation showcases proteomics services and kits from Cell Signaling Technology, including technologies that leverage antibodies specific for post-translational modifications (PTMs) to analyze activities in multiple signaling pathways. Various enrichment approaches are compared in the context of T Cell Receptor signaling, and validation of the methodologies are presented. Learn about our proteomics services: https://www.cellsignal.com/contents/services/ptmscan-sup-sup-services/ptmscan-services Learn about our proteomics kits: https://www.cellsignal.com/common/content/content.jsp?id=proteomics-kits Featured Speaker: Matthew Stokes, PhD Assoc. Director Proteomics at Cell Signaling Technology Chapters: 0:20 About CST 1:13 PTMScan® Services 3:30 Motif Antibodies for PTMs 4:33 The PTMScan Method 6:24 Phosphorylation Enrichment Methods 10:31 PTMScan Analysis: TCR Signaling 14:12 Validation of PTMScan data 15:06 Targeted Assays 17:37 Proteomics Conclusions ?About CST®: Cell Signaling Technology (CST) is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools to the biomedical research community. Our employees operate worldwide from our U.S. headquarters in Massachusetts, and our offices in the Netherlands, China, and Japan. https://cellsignal.com/about Cell Signaling Technology, CST, and PTMScan are registered trademarks of Cell Signaling Technology, Inc. #antibody #proteomics